Future thinking on carved stones

No abstract available

Scottish Archaeological Research Framework: Future Thinking on Carved Stones

No abstract available

An eighth-century inscribed cross-slab in Dull, Perthshire

The discovery of a cross-inscribed slab with an inscription is described. Because of its importance it was decided to invite specialist comment on this piece of sculpture and to publish it in advance of the full report on the excavations. The form of the monument, its inscription and archaeological context are considered. The text appears to consist of a Gaelic personal name. The script is a form of geometrical lettering which can be dated to the opening quarter of the eighth century. The form of the cross has Columban associations which sit well with place-name and other evidence which points to Dull having been a monastery founded from Iona by c. AD 700

Future Thinking on Carved Stones in Scotland

An introduction to the newly published, online Future Thinking on Carved Stones in Scotland: A Research Framework resource

Early literacy and multilingualism in Ireland and Britain

No abstract available

Neutron scattering for STRUCTURAL BIOLOGY

The past two decades have seen explosive growth in research on structural molecular biology. High-throughput techniques for determining biological structures are yielding large amounts of information about atomic-, cellular-, and tissue-scale organization. Advances are driven by modern high-brilliance synchrotron sources, synchrotron-based full-field x-ray microscopy and tomography, free-electron lasers, and cryoelectron microscopy facilities. The scientific landscape is changing at a remarkable pace with increasing emphasis being placed on interdisciplinary and multi-technique approaches. Neutron scattering facilities around the globe are expanding their capabilities to provide unique and complementary insights about biological systems

Nanoscale structure and dynamics of model membrane lipid raft systems, studied by neutron scattering methods

Quasi-elastic neutron scattering (QENS) and small angle neutron scattering (SANS), in combination with isotopic contrast variation, have been used to determine the structure and dynamics of three-component lipid membranes, in the form of vesicles, comprising an unsaturated [palmitoyl-oleoyl-phosphatidylcholine (POPC) or dioleoyl-phosphatidylcholine (DOPC)], a saturated phospholipid (dipalmitoyl-phosphatidylcholine (DPPC)), and cholesterol, as a function temperature and composition. SANS studies showed vesicle membranes composed of a 1:1:1 molar ratio of DPPC:DOPC:cholesterol and a 2:2:1 molar ratio of DPPC:POPC:cholesterol phase separated, forming lipid rafts of ∼18 and ∼7 nm diameter respectively, when decreasing temperature from 308 to 297 K. Phase separation was reversible upon increasing temperature. The larger rafts observed in systems containing DOPC are attributed to the greater mis-match in lipid alkyl chains between DOPC and DPPC, than for POPC and DPPC. QENS studies, over the temperature range 283–323K, showed that the resulting data were best modelled by two Lorentzian functions: a narrow component, describing the “in-plane” lipid diffusion, and a broader component, describing the lipid alkyl chain segmental relaxation. The overall “in-plane” diffusion was found to show a significant reduction upon increasing temperature due to the vesicle membranes transitioning from one containing rafts to one where the component lipids are homogeneously mixed. The use of different isotopic combinations allowed the measured overall reduction of in-plane diffusion to be understood in terms of an increase in diffusion of the saturated DPPC lipid and a corresponding decrease in diffusion of the unsaturated DOPC/POPC lipid. As the rafts are considered to be composed principally of saturated lipid and cholesterol, the breakdown of rafts decreases the exposure of the DPPC to cholesterol whilst increasing the exposure of cholesterol to unsaturated lipid. These results show the sensitivity of lipid diffusion to local cholesterol concentration, and the importance of considering the local, rather that the global composition of a membrane when understanding the diffusion processes of lipids within the membrane. The novel combination of SANS and QENS allows a non-intrusive approach to characterize the structure and dynamics occurring in phase-separated model membranes which are designed to mimic the lateral heterogeneity of lipids seen in cellular membranes–a heterogeneity that can have pathological consequences

Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris

Deuteration of biomolecules has a great impact on both quality and scope of neutron scattering experiments. Cholesterol is a major component of mammalian cells, where it plays a critical role in membrane permeability, rigidity and dynamics, and contributes to specific membrane structures such as lipid rafts. Cholesterol is the main cargo in low and high-density lipoprotein complexes (i.e. LDL, HDL) and is directly implicated in several pathogenic conditions such as coronary artery disease which leads to 17 million deaths annually. Neutron scattering studies on membranes or lipid-protein complexes exploiting contrast variation have been limited by the lack of availability of fully deuterated biomolecules and especially perdeuterated cholesterol. The availability of perdeuterated cholesterol provides a unique way of probing the structural and dynamical properties of the lipoprotein complexes that underly many of these disease conditions. Here we describe a procedure for in vivo production of perdeuterated recombinant cholesterol in lipid-engineered Pichia pastoris. Using flask and fed-batch fermenter cultures in deuterated minimal medium perdeuteration of the purified cholesterol was verified by mass spectrometry and its use in a neutron scattering study was demonstrated using neutron reflectometry